The increase of NADH fluorescence lifetime is associated with the metabolic change during osteogenic differentiation of human mesenchymal stem cells (hMSCs)
نویسندگان
چکیده
Background Fluorescence lifetime of NADH had been proposed to use as an intrinsic biomarker for monitoring cellular metabolism. In our pervious studies, we have demonstrated that NADH lifetime of hMSCs increase gradually with time of osteogenic differentiation. In this study, we performed NADH lifetime measurement of hMSCs from a different donor and studied the association with several metabolic indices such as ATP level, oxygen consumption and lactate release. We also measured the quantity of Complex I, III, IV and V during hMSC differentiation.
منابع مشابه
Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies.
The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs dur...
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